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Promoter Sequence

Make a list o...

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Make a list of plasmids which make sticky ends and which make blunt ends?

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Q. Which of the following enzyme creates a homonucleotide overhang at the

3^{'}

-OH end of the DNA? It can also make blunt ends sticky by adding poly AA and poly TT.

Q. Match the List-I with List-II and select the correct answer.

List-I	List-II
a. Recombinant DNA	$1$ . Marker gene-reporter gene
b. Agro bacterium	$2$ . DNA lipase
c. Sticky ends	$3$ . T-DNA
d. Plasmids	$4$ . Restriction enzymes

Q. The restriction enzymes used in recombinant DNA technology that make staggered cuts in DNAleaving sticky ends is/are

Q. The restriction enzyme(s) used in recombinant DNA technology making staggered cuts in DNA leaving sticky ends is/are

Q. Assertion: Sticky ends forms hydrogen bonds with their complementary cut counterparts.
Reason: In recombinant DNA technology, sticky end producing restriction enzymes are preferred over blunt ends producing restriction enzymes.

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BIOLOGY

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Promoter Sequence

Standard XII Biology

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