Question

Mention two approaches used while sequencing human genome.

Answer 1

BAC to BAC sequencing	whole genome shotgun sequencing
The BAC to BAC approach first creates a crude physical map of the whole genome before sequencing the DNA. Constructing a map requires cutting the chromosomes into large pieces and figuring out the order of these big chunks of DNA before taking a closer look and sequencing all the fragments.	The shotgun sequencing method goes straight to the job of decoding, bypassing the need for a physical map. Therefore, it is much faster.

Two approaches have been used to sequence the genome. They differ in the method they use to cut up the DNA, assemble it in the correct order and whether they map chromosomes before decoding the sequence. First, there was the BAC to BAC approach. A second newer method is called whole genome shotgun sequencing.