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Ligase

Name an eukar...

Question

Name an eukaryotic organism that has plasmids, and can be used as a host in gene cloning experiments.

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Solution

Yeast is the eukaryotic organisms that has plasmids and can be used as host in gene cloning experiments

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Q. Assertion: Ti plasmids are used to transfer genes to the host cells
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I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?

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II. Insertion of eukaryotic DNA into the vector.
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I. Introduction of cloning vector into cells.
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III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
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is a cloning vector that lacks antibiotic resistance genes.

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