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Dinoflagellates

Okk sir you a...

Question

Okk sir you are saying that eukaryotes do not have restriction enzymes okk now please explain me that how does the Ti plasmid insert the T DNA into plant cell chromosomes...????

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Solution

Ti Plasmid is present in a bacterium called Agrobacterium tumefaciens. Ti plasmid has a segment called T-DNA,
T-DNA can cut and attach to the plant DNA on its own with the help of enzymes.It has genes which can produces these enzymes for cutting and joing to plant DNA.

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Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
All of the following statements is true about restriction enzymes except

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement 2: Presence of insert results into insertional inactivation of -galactosidase enzyme and the colonies do not produce any colour.

Q. Sir do eukaryotes have restriction endonucleases?????
If no why ????

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