Question

One of the following can be visualized under fluorescent microscope.

• A. Chloroplast
• B. Actin filaments
• C. Nucleus
• D. Both (A) and (B)

Answer 1

The correct option is D Both (A) and (B)

Chlorophyll exhibits fluorescence. Chlorophyll fluorescence is light re-emitted by chlorophyll molecules during return from excited state to non-excited states and used as an indicator of photosynthetic energy conversion in higher plants.
In fixed cells, actin structures can be visualized by antibodies or by fluorescent phalloidin or electron microscopy. Correctly designed fluorescent phalloidin only bind to the native quaternary structure of F-actin and, thus, also have a less background.