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Recombinant DNA Technology

One of the fo...

Question

One of the following is not a step involved in recombinant DNA technology

A

cuts made in a DNA molecule with the help of restriction enzymes

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B

joining of DNA fragments with the vector

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C

cloning of hybrid DNA into the host

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D

karyotyping of the host DNA

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Solution

The correct option is D karyotyping of the host DNA
Recombinant DNA technology involves recombination of DNA molecules from two different organisms and cloning of this recombinant DNA.
A karyotype is the number and appearance of chromosomes in the nucleus of a eukaryotic cell. While karyotyping is a test that evaluates the number and structure of a person's chromosomes in order to detect abnormalities.
So, the correct option is 'Karyotyping of the host DNA'.

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Q. Identify the correct sequence/steps involved in recombinant DNA technology:
A) Culturing the host cells in a medium at large scale
B) Isolation of DNA
C) Isolation of a desired DNA fragment
D) Transferring the recombinant DNA into the host
E) Ligation of the DNA fragment into a vector
F) Fragmentation of DNA by restriction endonucleases
G) Extraction of the desired product

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. Recombinant DNA technology involves several steps in specific sequence. Find out the correct sequence.

a. Fragmentation of DNA
b. Culturing the host cells in a medium at large scale
c. Ligation of DNA fragment into a vector
d. Extraction of the desired product
e. Isolation of DNA
f. Isolation of desired DNA fragment
g. Transferring the recombinant DNA into the host

Q. Arrange the given steps involved in recombinant DNA technology in the correct order.

A. Amplification of desired DNA sequence (gene of interest) with PCR modification
B. Ligation of gene of interest with the vector
C. Removal of RNA and proteins to purify DNA
D. Insertion of recombinant DNA into the host
E. Treatment of cell with cell wall digesting enzyme followed by disruption of the cell membrane
F. Digestion of DNA of interest and vector with suitable restriction enzyme

Q. Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?

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