Please tell me what are stains and their types with function like safrannin is used for staining plant cells etc....
Please tell me what are stains and their types with function like safrannin is used for staining plant cells etc....
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells. Given below are some common stains and their uses and the colour they show up as:
Iodine: Stains carbohydrates in plant and animal specimens brown or blue- black.Stains glycogen red.
Methylene blue: Stains acidic cell parts (like nucleus) blue. Use on animal, bacteria and blood specimens. Can be used as a substitute for Janis B green.
Eosin Y: Stains alkaline cell parts (like cytoplasm) pink. Use on plants,animals and blood. Can be used as a substitute for Congo Red and Carmine.
Safranin : Mainly used for sections of plant tissues, stains red
Toluidene blue: Stains acidic cell parts (like nucleus) dark blue. Good to show mitosis in plant cells.
Wright’s stain: Stains red blood cells pink/red.
Leishman’s stain: Stains nucleus of WBC blue and blood cells pink
Crystal Violet: Stains bacteria purple
Aceto-orcein: Biological stain for chromosomes and connective tissue.
Sudan III: Biological stain used as a lipid indicator.
Given below are some common stains and their uses and the colour they show up as:
Iodine: Stains carbohydrates in plant and animal specimens brown or blue- black.Stains glycogen red.
Methylene blue: Stains acidic cell parts (like nucleus) blue. Use on animal, bacteria and blood specimens. Can be used as a substitute for Janis B green.
Eosin Y: Stains alkaline cell parts (like cytoplasm) pink. Use on plants,animals and blood. Can be used as a substitute for Congo Red and Carmine.
Safranin : Mainly used for sections of plant tissues, stains red
Toluidene blue: Stains acidic cell parts (like nucleus) dark blue. Good to show mitosis in plant cells.
Wright’s stain: Stains red blood cells pink/red.
Leishman’s stain: Stains nucleus of WBC blue and blood cells pink
Crystal Violet: Stains bacteria purple
Aceto-orcein: Biological stain for chromosomes and connective tissue.
Sudan III: Biological stain used as a lipid indicator.
