Question

Read the following statements with respect to ELISA and select the correct option. (T-true, F-false)
I. ELISA test is employed as the first and most basic test for an individual to detect HIV.
II.Expanded form of ELISA is Enzyme Linked Immunosorbent Assay and is used to identify specific proteins.
III. ELISA detects DNA in HIV-positive patients.

• A. I - T, II - F, III - T
• B. I - F, II - T, III - F
• C. I - T, II - T, III - F
• D. I - T, II - F, III - F

Answer 1

The correct option is C I - T, II - T, III - F

ELISA is the basic assay technique, known as Enzyme Linked Immuno-sorbent Assay.

ELISA is used to detect and quantify substances, including antibodies, antigens, proteins, glycoproteins, and hormones. The detection of these substances is accomplished by complexing antibodies and antigens to produce a measurable result. Hence, ELISA is based on the principle of antigen-antibody interaction. ELISA can provide a useful measurement of antigen-antibody concentration. It is rapid, quick and requires a blood sample of the patient.

ELISA testing is used to diagnose HIV infection. It helps to examine the presence of antibodies synthesised against HIV in the body.

ELISA test is employed as the first and most basic test for an individual to detect HIV. If a person tests positive for HIV on the ELISA test, the provider orders the Western blot test to confirm HIV infection.

ELISA does not detect DNA in the blood of a patient.