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Question

Restriction endonuclease, an enzyme used in genetic engineering, is employed for:

A
Probing exons
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B
Cutting double-stranded DNA
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C
Cutting single-stranded DNA
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D
Joining strands of DNA
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Solution

The correct option is B Cutting double-stranded DNA
A restriction enzyme is an enzyme that cuts double-stranded DNA at or near specific recognition nucleotide sequences known as restriction sites. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e., each strand) of the DNA double helix.

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