Question

Restriction endonuclease, in DNA fingerprinting, carries out the following process.

• A. Fragmentation of DNA
• B. Getting copies of DNA
• C. Loading DNA on agaroseplate
• D. Synthesis of DNA

Answer 1

Answer: (A)

Fragmentation of DNA

• PCR is a technique used to amplify the DNA molecules present in a small sample in order to obtain a large amount of DNA sequence for the test.
• This is followed by restriction fragment length polymorphism (RFLP). The enzyme restriction endonuclease is used for fragmentation of DNA.
• RFLP analysis is used to identify the repeated sequences by detecting a specific sequence pattern to the Variable Number Tandem Repeats which becomes that individual's DNA profile. This is the basis of genetic fingerprinting.

Thus, the correct answer is option A.