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Question


Restriction enzymes were discovered by microbiologists who were studying the host range of infectious bacteriophage. The term restriction refers to the fact that different strains of bacteria are resistant to bacteriophage infection if they contain the appropriate endonucleases that can destroy infecting DNA. Observe this figure and answer the following questions.

What are structures labeled A and B respectively?
223079_bd65b760360846d1919f66a3df1d0738.png

A
A is a restriction endonuclease and B is a ligase
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B
A is a ligase and B is a methylase
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C
A is a restriction endonuclease and B is a methylase
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D
A is a polymerase and B is a methylase
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Solution

The correct option is C A is a restriction endonuclease and B is a methylase
  • A restriction enzyme restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites.
  • Methylase is an enzyme that adds a methyl group to the adenine of the sequence 5'-GATC-3' in newly synthesized DNA.
  • Hence A is a restriction endonuclease and B is a methylase in structure labelled A and B respectively
  • So the correct answer is 'A is a restriction endonuclease and B is a methylase'.


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