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Fermenters

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Question

Select proper option showing name and aim of the given figure.

A

PCR, As a selectable marker

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B

PCR, To cut the desired DNA segment

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C

Bioreactor, Provides the optimal conditions for large scale production of desired protein.

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D

Bioreactor, Produces Recombinant DNA possessing antibiotic resistance gene

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Solution

The correct option is C Bioreactor, Provides the optimal conditions for large scale production of desired protein.
The given figure is of bioreactor. It is an apparatus that provides the optimal conditions for a biological reaction to be carried out on large scale. Under optimum conditions, it can be used for the large scale production of desired protein.
Thus, the correct answer is 'Bioreactor, Provides the optimal conditions for large scale production of desired protein.'

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Q. Study the following statements regarding recombinant DNA technology and select the incorrect ones

(i) Taq polymerase extends the primers using the nucleotides provided in the reaction

(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology

(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis

(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium

(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture

(vi) Downstream processing is one of the steps of polymerase chain reaction

Q. Tom was trying to isolate a human gene (X) responsible for producing an enzyme (Y). His target was to synthesize Y on a large scale for commercial utility. Mention the correct order of the steps that he needs to perform in order to isolate his gene of interest.
I. Obtaining cell sample from desired tissue
II. Separation of desired DNA by gel electrophoresis
III. Cell lysis
IV. Amplification of desired DNA sequence by PCR
V. Isolation of genomic DNA
VI. Identification of desired band, cutting and elution of DNA from gel

Q. Given below are the steps of recombinant DNA technology.

(A) Introduction of desired gene into the host
(B) Identification of desired gene
(C) Transfer of desired gene to the progeny
(D) Isolation of desired gene
(E) Maintenance of introduced DNA into the host

Choose the correct sequence of events.

Q. Identify the correct sequence/steps involved in recombinant DNA technology:
A) Culturing the host cells in a medium at large scale
B) Isolation of DNA
C) Isolation of a desired DNA fragment
D) Transferring the recombinant DNA into the host
E) Ligation of the DNA fragment into a vector
F) Fragmentation of DNA by restriction endonucleases
G) Extraction of the desired product

Q. The given flow chart depicts the steps to transfer a desirable gene of interest into a plant.Isolation of desirable gene using restriction endonucleases and gel electrophoresis.
Regeneration of plants from the transformed cells to get transgenic plants.
Identify the missing steps (A, B and C) with regard to following statements and select the correct option.(i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule(ii) Selection of transformed cells(iii) Transferring the recombinant DNA molecules to the target cells

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