Fermenters

Q. Read the following statements and choose the most appropriate option.
I. ____A____ are bombarded with high velocity micro-particles of ___B____ coated with DNA in a method known as biolistics.
II. Recombinant DNA is directly injected into the nucleus of an animal cell in the method called as___C______.

1. A - Plant cells, B - gold, C - gene gun
2. A - Plant cells, B - gold/ aluminium , C - electroporation
3. A - Animal cells, B - aluminium/ tungsten, C - electroporation
4. A - Plant cells, B - gold/ tungsten, C - microinjection

Q.

What are the 2 types of bioreactors?

Q.

Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors to have over shake flasks?

Q. Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?

Q. A device in which substances are treated to stimulate transformation by living cells is called

1. assimilator
2. agitator
3. bioreactor
4. digester

Q. What is bioreactors?

Q.

Arrange the following in the decreasing order (most important first) of their importance, for the welfare of human society. Give reasons for your answer.

Biogas, Citric acid, Penicillin and Curd

Q. Separation and purification by filtration, centrifugation of culture broth are a part of

1. Screening for recombinants and downstream processing
2. Upstream and downstream processing
3. Upstream processing
4. Downstream processing

Q. What is bioreactor? Explain its functioning.

Q. What is the advantage of sparged stirred-tank bioreactor?

Q. What is the main function of bioreactors?

Q. Draw a neat labelled diagram of stirred tank bioreactor.

Q. Label the parts of a bioreactor:

1. Impeller
2. Extraction Vent
3. Temp. Control
4. Cooling Jacket

Q. How does stirring the broth in the bioreactor help?

1. Avoids contamination
2. Causes the cells to declump
3. Improves aeration among cells
4. Decreases frothing

Q. Describe briefly the bioreactors.

Q. Name the type of bioreactor shown. Write the purpose for which it is used.

Q. A device in which large volume of living cells are cultured in order to get a specific product is called

1. Agitator
2. Bioreactor
3. Assimilator
4. PCR

Q. Describe briefly the followings:
(a) Origin of replication
(b) Bioreactors
(c) Downstream processing

Q. Stirred-tank bioreactor has been designed for

1. purification of the product
2. ensuring anaerobic conditions
3. oxygen availability throughout the bioreactor
4. addition of preservatives to the product

Q. What are the advantages of bioreactor which uses continuous culture system rather than batch culture?

Q.

Arrange the following in the decreasing order (most important first) of their importance, for the welfare of human society. Give reasons for your answer.

Biogas, Citric acid, Penicillin and Curd

Q. Name two commonly used bioreactors. State the importance of using a bioreactor.

Q. What are fermentors?

Q. Assertion :In a bioreactor, temperature, pH, substrate, salts, vitamins, and oxygen are maintained. Reason: They are added to maintain the proper growth of the organism and to achieve desired product optimally.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. Which of the following statements is not true for stirred tank fermentation?

1. Buffer needed to control
2. Batch and feed possible
3. Control dissolved oxygen
4. Easy in process sampling

Q. Jatropha is a

1. Fibre crop
2. Biodiesel crop
3. Biopetrol crop
4. Food crop

Q.

how do u define bioactive molecules?

Q. Which one of the following is being utilized as a source of biodiesel in the Indian countryside?

1. Euphorbia
2. Beetroot
3. Sugracane
4. Pongarnia

Q. Observe the sketch of stirred-tank bioreactor and label the parts A, B, C and D.

Q. Write two significance of bioreactors.