Question

Select the options which will result in expression of a human gene into functional proteins.
I. cDNA for the protein is inserted into the genome of an E. coli host.
II. mRNA is inserted into the genome of an E. coli host.
III. Gene is inserted into the genome of Saccharomyces.
IV. Gene is inserted into the genome of E. coli host.

• A. I and II
• B. II and III
• C. I and III
• D. Only III

Answer 1

The correct option is C I and III

cDNA (complementary DNA) is stripped of introns. When this is inserted for a protein into the genome of an E.coli host, it results in the expression of the gene. Insertion of gene into the genome of Saccharomyces also results in the expression of the gene.

mRNA is single stranded and cannot be inserted into the genome.

Eukaryotic human genes can be inserted into a prokaryote like E.coli but they cannot be expressed as functional proteins.