Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plated on two different media - one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector

will grow on both tetracycline containing and ampicillin containing media

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will not grow on either tetracycline containing or ampicillin containing media

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will not grow on tetracycline, but not on ampicillin-containing media

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will grow on ampicillin but not on tetrecycline-containing media

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Solution

The correct option is A will grow on both tetracycline containing and ampicillin containing media
The transformed cells grow on both tetracycline containing and ampicillin containing media. This is because the foreign DNA fragment is attached to Cla I site and so, it does not cause the disintegration of either amp or tet gene, thus providing resistance against both the antibiotics.

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