Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plated on two different media one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector

Will grow on both tetracycline containing and ampicillin containing media

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Will not grow on either tetracycline containing or ampicillin containing media

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Will grow on tetracycline but not on ampicillin containing medium

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Will grow on ampicillin but not on tetracycline containing medium

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Solution

The correct option is B Will grow on both tetracycline containing and ampicillin containing media
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Plasmid pBR322 confers resistance to ampicillin and tetracycline. Since, the foreign DNA fragment is attached to Cla 1 site, the transformant will be observed on both the plates.

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Q. Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plated on two different media-one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector

Q. Some of foreign DNA fragment is attached to

C l a I

site of

p B R 322.

This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plates on two different media - one containing ampicillin and the other containing tetracycline. They transformed cells containing the recombinant vector :

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