Question

Some of the steps involved in the production of humulin are given. Choose the correct sequence (i) Synthesis of gene (DNA) for human insulin artificially (ii) Culturing recombinant E.coli in bioreactors (iii) Purification of humulin (iv) Insertion of human insulin gene into plasmid (v) Introduction of recombinant plasmid into E. coli (vi) Extraction of recombinant gene product from E. coli.

• A. ii, i, iv, iii, v, vi
• B. i, iii, v, vi, ii, iv
• C. i, iv, v, ii, vi, iii
• D. iii, v, ii, i, vi, iv

Answer 1

The correct option is C i, iv, v, ii, vi, iii

The correct sequence is

1.Synthesis of the gene for human insulin artificially.

2.Insertion of human insulin.

3.Introduction of recombinant plasmid into E.coli

4. Culturing recombinant E.coli in bioreactors.

5.Extraction of a recombinant gene product from E.

6.Purification of Humulin.

So, the correct option is 'i, iv, v, ii, vi, iii'.