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Extraction of Genomic DNA

The acid used...

Question

The acid used for preliminary separation of biomolecules in a living tissue is

A

Trichlorobenzoic acid

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B

Benzoic acid

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C

Acetic acid

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D

Trichloroacetic acid

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Solution

The correct option is D Trichloroacetic acid
In order to study the different biomolecules, the living tissues are ground in trichloroacetic acid using a mortar and a pestle to form a thick soup. It is then strained to obtain two fractions: acid soluble and acid insoluble fraction. Chemicals present in both the fractions are further separated by various analytical techniques and identified.

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Q. The acid used for preliminary separation of biomolecule in a living tissue is

Take a living tissue, grind it in trichloroacetic acid using pestle and mortar, and then strain it, you would obtain two fractions: acid-soluble and acid-insoluble fraction. Acid-insoluble fraction does not contain

Q. The table below shows the pH values of some chemicals.

Chemical pH
hydrochloric acid 2
ammonium hydroxide 10
sulphuric acid 1
acetic acid 5

Which of the above is the strongest acid?
(a) ammonium hydroxide
(b) acetic acid
(c) hydrochloric acid
(d) sulphuric acid

Q. In the figure given below , percentage composition of biomolecules in living tissue is shown. Phospholipids are likely to be obtained in the fraction labelled.

Q. Assertion :Arachidonic acid is a

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carbon polyunsaturated fatty acid Reason: Insulin and sholesterol will be obtained in acid soluble fraction during living tissue analysis.

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