Question

The correct sequence in the tissue culture technique is ______________.

• A. Inoculation - explant incubation - callus formation - organogenesis - preparation of medium - transferred to field
• B. Preparation of medium - inoculation - explant incubation - callus formation - organogenesis - transferred to field
• C. Explant incubation - preparation of medium - inoculation - organogenesis - callus formation - transferred to field
• D. Preparation of medium - inoculation - explant incubation - organogenesis - callus formation - transferred to field

Answer 1

The correct option is B Preparation of medium - inoculation - explant incubation - callus formation - organogenesis - transferred to field

The flowchart shows the steps for plant tissue culture:

Intact Plant - Excision of explant - proper trimming of explant - surface sterilization - sterilization of glassware - explant washed in sterilized distilled water - culture medium is sterilized in Autoclave - inoculation of explant in the culture medium under aseptic conditions - incubation under suitable condition for proper culture - subculture for obtaining desired results - plantlet transfer.

So, option B is the correct option.