Question

The correct sequence of different steps of PCR

• A. Annealing $\to$ Denaturation $\to$ Extension
• B. Denaturation $\to$ Extension $\to$ Annealing
• C. Denaturation $\to$ Annealing $\to$ Extension
• D. Extension $\to$ Denaturation $\to$ Annealing

Answer 1

The correct option is C Denaturation $\to$ Annealing $\to$ Extension

Denaturation - Separation of parent DNA strands
Annealing - Attachment of the primers to the template DNA
Extension - Formation of new DNA with Taq polymerase