1
Question
The DNA fragments separated on an agarose gel can be visualised after staining with
The DNA fragments separated on an agarose gel can be visualised after staining with
Open in App
Solution
The correct option is A Ethidium bromide
Visualisation of DNA in agarose gel
Visualisation of DNA in the agarose gel is done in the following way:
- To visualise DNA in the agarose gel, a positively charged dye ethidium bromide (EtBr) is added in the agarose gel or electrophoresis buffer.
- This dye intercalates itself into the DNA backbone and gives orange fluorescence upon exposure to the UV light.
- Thus, DNA is visualised by exposure to UV light as orange coloured bands on the agarose gel.
Acetocarmine
Acetocarmine is a DNA specific stain produced by mixing carmine with glacial acetic acid. It is used for the visualisation of supercoiled chromosomes in the different stages of mitosis.
Aniline blue
Aniline blue is a mixture of methyl blue and water blue. It is used to stain collagen.
Bromophenol blue
Bromophenol blue is a pH indicator. It is used as a tracking dye during agarose or polyacrylamide gel electrophoresis to monitor the progress of agarose gel electrophoresis.
Final answer
c. Ethidium bromide.
Visualisation of DNA in agarose gel
Visualisation of DNA in the agarose gel is done in the following way:
- To visualise DNA in the agarose gel, a positively charged dye ethidium bromide (EtBr) is added in the agarose gel or electrophoresis buffer.
- This dye intercalates itself into the DNA backbone and gives orange fluorescence upon exposure to the UV light.
- Thus, DNA is visualised by exposure to UV light as orange coloured bands on the agarose gel.
Acetocarmine is a DNA specific stain produced by mixing carmine with glacial acetic acid. It is used for the visualisation of supercoiled chromosomes in the different stages of mitosis.
Aniline blue
Aniline blue is a mixture of methyl blue and water blue. It is used to stain collagen.
Bromophenol blue
Bromophenol blue is a pH indicator. It is used as a tracking dye during agarose or polyacrylamide gel electrophoresis to monitor the progress of agarose gel electrophoresis.
Final answer
c. Ethidium bromide.
Suggest Corrections
0
Join BYJU'S Learning Program
Explore more
Join BYJU'S Learning Program
