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Question
The linking of antibiotic resistance gene with the plasmid vector became possible with which of the following enzymes?
The linking of antibiotic resistance gene with the plasmid vector became possible with which of the following enzymes?
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Solution
The correct option is C DNA ligase
The enzymes DNA polymerase is used for DNA synthesis, Exonucleases and endonucleases are used for excision of DNA or RNA strands. DNA ligase is the enzyme used to ligate the attached antibiotic resistance gene in the plasmid vector. It catalyses the joining of DNA strands with the help of formation of phosphodiester bonds between two DNA strands and helps in the complete insertion of antibiotic resistance gene In a plasmid vector.
The enzymes DNA polymerase is used for DNA synthesis, Exonucleases and endonucleases are used for excision of DNA or RNA strands. DNA ligase is the enzyme used to ligate the attached antibiotic resistance gene in the plasmid vector. It catalyses the joining of DNA strands with the help of formation of phosphodiester bonds between two DNA strands and helps in the complete insertion of antibiotic resistance gene In a plasmid vector.
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