Question

The most useful plasmid for cloning is

• A. pBR 323
• B. pTM 223
• C. pBR 222
• D. pBR 322

Answer 1

Answer: (D)

pBR 322

• A cloning vector is a small piece of DNA, which carries the desired gene from one cell to another for cloning.
• There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids.
• pBR322 is a plasmid and is one of the first widely used E. coli cloning vectors.
• It was created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco.

Hence, Option D is correct.