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Question

The number of nucleotides on the mRNA, ATP utilized, peptide bond formed, translocations and ribosomes required to synthesize a with polypeptide with 500 amino acids

A
1500, 499, 500, 500 & 500
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B
1503, 500, 499, 499 & 1
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C
1530, 500, 499, 500 & 1
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D
1500, 499, 499, 500 & 499
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Solution

The correct option is B 1503, 500, 499, 499 & 1

1. On the mRNA three nucleotides form a codon for the amino acid. It has one start codon, AUG for methionine, all remaining codon for the polypeptide chain and one of three stop codon UAA, UAG, UGA which do does not code for any amino acid. So, for 500 amino acid, the number of nucleotides are 500*3 = 1500 + 1 stop codon=1503 nucleotides.

2. 1 molecules of ATP are converted into AMP + 2Pi to activate 1 amino acid, so for 500 amino acid 500 ATP utilized.

3. A peptide bond is an amide type of chemical bond linking two consecutive alpha- amino acids from C1 of one alpha-amino acid and N2 of another along a peptide or protein chain.

So, for the 500 amino acid to join, 499 peptide bond is required.

4. Translocation denotes the simultaneous movement of two tRNAs together with the mRNA by one codon on the ribosome. So, for the 500 amino acid, 499 translocations are required.

5. During translation, ribosomal subunits assemble together like a sandwich on the strand of mRNA, where they proceed to attract tRNA molecules tethered to amino acids (circles). A long chain of amino acids emerges as the ribosome decodes them RNA sequence into a polypeptide or a new protein. 1 ribosome is used for the translation.

So, the correct answer is “Nucleotides=1503, ATP= 500, Peptide Bond=499, Translocations=499 and Ribosomes= 1”


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