Question

The pH of nutrient medium in plant tissue culture is adjusted between

• A. $3-4$
• B. $4.1-4.8$
• C. $5-5.8$
• D. $6-7$

Answer 1

Answer: (C)

$5-5.8$

The optimal pH for most tissue cultures is in the range of 5.0-6.0 as at pH higher than 7. and lower than 4.5 the plant cells stop growing in cultures. pH above 6.0 gives the medium hard appearance while pH below 5.0 does not allow gelling of the medium. Thus the correct answer is option C.