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Question
The restriction site recognised by enzyme EcoRI is
The restriction site recognised by enzyme EcoRI is
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Solution
The correct option is C GAATTC
Restriction enzymes like EcoRI cut through the DNA strands at nucleotides that are not exactly opposite to each other. This creates DNA fragments with one nucleotide strand that overhangs at the end. This overhanging nucleotide strand is called a sticky end because it can easily bond with complementary DNA fragments. It creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry.
Restriction enzymes like EcoRI cut through the DNA strands at nucleotides that are not exactly opposite to each other. This creates DNA fragments with one nucleotide strand that overhangs at the end. This overhanging nucleotide strand is called a sticky end because it can easily bond with complementary DNA fragments. It creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry.
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