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B
Steam at 100oC for 1 hour.
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C
Steam under pressure at 121oC for 15 minutes.
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D
Steam at 121oC for 5 minutes.
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Solution
The correct option is D Steam under pressure at 121oC for 15 minutes. A widely used method for heat sterilization is the autoclave. Steam heat has ability to penetrate microbes and kill them. Autoclaves commonly use steam heated to 121-134oC (250-273 F) for 15 minutes.