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Molecular Diagnosis

The technique...

Question

The technique of DNA fingerprinting involves
(A) Digestion of DNA by restriction enzymes
(B) Isolation of DNA
(C) Hybridisation
(D) Blotting
(E) Separation by electrophoresis
(F) autoradiography

Choose the correct order of the steps

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Q. Arrange the various steps of DNA fingerprinting technique in the correct order
(i) Separation of DNA fragments by electrophoresis
(ii) Digestion of DNA by restriction endonucleases
(iii) Hybridization using labeled VNTR probe
(iv) Isolation of DNA
(v) Detection of hybridized DNA fragments by autoradiography
(vi) Transferring the separated DNA fragments to nitrocellulose membrane

Q. Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the options given:
A. Electrophoresis of DNA fragments
B. Hybridization with DNA probe
C. Digestion of DNA by restriction endonucleases
D. Autoradiography
E. Blotting of DNA fragments nitrocellulose membrane

Q. Some of the steps of DNA fingerprinting are given below. Identify the correct sequence from the options given.

A

. Electrophoresis of DNA fragments

B

. Hybridisation with DNA probe

C

. Digestion of DNA by RENs

D

. Autoradiography

E

. Blotting of DNA fragments to nitrocellulose membrane

Some of the steps of DNA fingerprinting are given below. Identify the correct sequence from the options given.

I. Electrophoresis of DNA fragments.

II. Hybridisation with DNA probe.

III. Digestion of DNA of RENs.

IV. Autoradiography.

V. Blotting of DNA fragments to nitrocelluose membrane.

Q. Select the correct sequences of steps in DNA fingerprinting involving Southern blot hybridisation using radiolabeled VNTR as probe.
I. Hybridisation using labelled VNTR probe.
II. Isolation of DNA.
III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon.
IV. Detection of hybridisation DNA fragments by autoradiography.
V. Separation of DNA fragments by electrophoresis.
VI. Digestion of DNA by restriction endonucleases.

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