Tom was trying to isolate a human gene (X) responsible for producing an enzyme (Y). His target was to synthesize Y on a large scale for commercial utility. Mention the correct order of the steps that he needs to perform in order to isolate his gene of interest.
I. Obtaining cell sample from desired tissue
II. Separation of desired DNA by gel electrophoresis
III. Cell lysis
IV. Amplification of desired DNA sequence by PCR
V. Isolation of genomic DNA
VI. Identification of desired band, cutting and elution of DNA from gel