Question

What are the requirements in Tissue culture?

• A. Hormones like auxin, cytokinin, agar-agar
• B. Inorganic salt, vitamin, amino acid only
• C. Carbon source like sucrose only
• D. All of these

Answer 1

Answer: (D)

All of these

• As traditional breeding techniques failed to keep pace with demand and to provide sufficiently fast and efficient systems for crop improvement, another technology called tissue culture got developed in which whole plants could be regenerated from explants.
• Explant i.e. any part of a plant taken out and grown in a test tube, under sterile conditions in special nutrient medium. A nutrient medium must provide a carbon source such as sucrose, inorganic salts, vitamins, amino acids and growth regulators like auxins, cytokinins etc.
• 
  
• So, the correct answer is 'All of these'