Question

What Is Asymmetric PCR?

Answer 1

Asymmetric PCR (PCR- Polymerase Chain Reaction):

1. Asymmetric PCR is a variant of PCR that is used to preferentially amplify one strand of the target one more than the other.
2. It is used to form single-stranded DNA from double-stranded DNA, which is then used for DNA sequencing etc.
3. The whole PCR process is the same as regular PCR except that the amount of primer for the targeted strand is much more than that of the non-targeted strand.
4. As the PCR reaction progresses, the lower concentration limiting primer gets used up.
5. After the depletion of the limiting primer, linear synthesis of the targeted single-stranded DNA is formed from the excess primer.