Transfer RNA (tRNA) is a small RNA molecule that plays an important role in protein synthesis.
Transfer RNA acts as a link (or adapter) between the messenger RNA (mRNA) molecule and the growing chain of amino acids that make up the protein.
The establishment of the peptide bond requires aminoacylation.
Three stages are necessary for protein synthesis: initiation, elongation, and termination.
The ribosome must first connect to mRNA at the start codon (AUG), which is always recognized by the initiator tRNA, in order for initiation to occur.
Because the formation of peptide bonds requires energy, the amino acids are activated in the presence of ATP and connected to their tRNA in the first step, this is known as tRNA charging or aminoacylation.