Template Strand Coding Strand
1. During transcription, only one of the two DNA strands is copied. 1. The non-template strand is known as the coding strand.
This is called the template strand because it acts as a template Because the coding strand and the mRNA formed from the
for the mRNA that is being assembled by the ribosomes. The template strand are both complementary to the template strand,
sequence of the mRNA is complementary to the sequence of the they will have the same sequence. The only exception is that
template strand. For example, a cytosine on the DNA template wherever there is a thymine in the DNA coding strand, there will
strand pairs with a guanine on the mRNA strand, and an adenine be a uracil in the mRNA. Remember, RNA has a uracil base instead
pairs with a uracil. of the thymine base that is found in DNA.
2. Template strand is that strand which has the sequence 2. It is that DNA strand which has sequence similar to RNA formed
complementry to that of the newly transcribed mRNA. after transcription except for the change that thymine is present
in place of uracil.
3. The direction of this strand is 3'→5'. 3. Coding starnd has the polarity 5'→3'
4. This is also called as antisense strand. 4. It is also called sense strand or non template strand.