What is the enzyme used to anneal the sticky ends of a plasmid to an inserted gene?

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Solution

Recombinant DNA technology:
Recombinant DNA technology (RDT) is a widely used technique in molecular biology, which involves the joining of two DNA molecules and inserted in to host for the production of the desired product.
So this RDT first involves the digestion of DNA molecules using restriction enzymes, which give sticky ends.
The sticky ends allow the insertion of foreign DNA into the host cell.
Plasmids are also digested by using the same restriction enzyme giving the same sticky ends.
DNA Ligase :
To join sticky ends DNA ligase enzyme is used which acts by catalyzing the formation of a phosphodiester bond.
DNA ligases are called Molecular glue since these enzymes are used during replication for joining phosphodiester backbone, used in RDT for joining sticky ends, and also used in damage and repair cells.
Hence, the DNA ligase enzyme is used to anneal the sticky ends of a plasmid to an inserted gene.

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