Which enzyme is used for the separation of genetic material?
A
Ligase
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B
Restriction endonuclease
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C
Hydrolase
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D
Amylase
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Solution
The correct option is D Restriction endonuclease Restriction enzymes are DNA-cutting enzymes found in bacteria and harvested from them for use. Because they cut within the molecule, they are often called restriction endonucleases. A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides. So, restriction endonucleases are used for separation of genetic material. Thus, option B is correct. Ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond.
Hydrolase is an enzyme that catalyzes the hydrolysis of a chemical bond.
Amylase is an enzyme that catalyzes the hydrolysis of starch into sugars.
So, the correct answer is 'Restriction endonuclease'