Question

Which of the following are the requirements for DNA fingerprinting?

1. Electron microscopy

2. Electrophoresis

3. Autoradiography

4. Southern blotting

5. Somatic cell fusion

• A. 1, 2 and 3
• B. 2, 3 and 4
• C. 1, 2, 3 and 4
• D. 1, 2, 3, 4 and 5

Answer 1

The correct option is B

2, 3 and 4

DNA fingerprinting, also known as DNA profiling is a technology combines few techniques such as electrophoresis, autoradiography and southern blotting. All these techniques are related to the separation of DNA fragments, their interactions and detection of their location on the gel.

Electron microscopy is used to magnify and observe the structures of cells while somatic cell fusion is a type of genetic modification in plants.