Question

Which of the following is not component of yeast artificial chromosome?

Answer 1

• Yeast artificial chromosomes are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces, which is then ligated into a bacterial plasmid.
  
• By inserting large fragments of DNA, from 100–1000 kb, the inserted sequences can be cloned and physically mapped using a process called chromosome walking.
  
• The primary components of a YAC are the ARS, centromere, and telomeres from Saccharomyces.
  
• Additionally, selectable marker genes, such as antibiotic resistance and a visible marker, are utilized to select transformed yeast cells.
  
• Without these sequences, the chromosome will not be stable during extracellular replication, and would not be distinguishable from colonies without the vector