Which of the following is not required in the preparation of a recombinant DNA molecules?

Restriction endonucleases

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DNA ligase

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DNA fragments

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E. coli

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Solution

The correct option is D E. coli
E. coli is not required for preparation of a recombinant DNA molecule rather it may be required for the expression of recombinant DNA-molecule. Restriction enzymes and DNA ligases can be used to make a stable recombinant DNA molecule with DNA fragments that has been spliced together from two different organisms.

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