Question

Which of the following is required in southern blotting?

• A. Probes
• B. Agarose gel
• C. Nitrocellulose membrane
• D. All of the above

Answer 1

The correct option is D All of the above

The Southern blotting technique was devised by E. M. Southern. In this technique, the DNA molecules are transferred and immobilised on a nitrocellulose or nylon membrane from an electrophoresis gel in such a way that the pattern present in the gel is reproduced on the membrane. It is followed by treatment with labelled probes which bind to complementary sequences in the sample.
So, Southern blotting requires all of the above(probes, agarose gel, and nitrocellulose membrane).