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Question
Which of the following stains is not used for staining chromosomes?
Which of the following stains is not used for staining chromosomes?
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Solution
The correct option is C Safranin
Basic fuchsin
Basic fuchsin is a cationic dye and can be used as a nuclear stain.
Safranin
Safranin is not usually used for staining chromosomes because it is used as a counterstain in some staining protocols.
Methylene green
Methylene green is a cationic or positively charged stain with affinity for DNA and hence, can be used in staining of chromosomes.
Carmine
Carmine is mixed with glacial acetic acid to produce acetocarmine (a DNA-specific stain) which is used for the observation of highly condensed chromosomes. Carmine is therefore used in staining slides containing various stages of mitosis.
Final answer b. Safranin
Basic fuchsin
Basic fuchsin is a cationic dye and can be used as a nuclear stain.
Safranin
Safranin is not usually used for staining chromosomes because it is used as a counterstain in some staining protocols.
Methylene green
Methylene green is a cationic or positively charged stain with affinity for DNA and hence, can be used in staining of chromosomes.
Carmine
Carmine is mixed with glacial acetic acid to produce acetocarmine (a DNA-specific stain) which is used for the observation of highly condensed chromosomes. Carmine is therefore used in staining slides containing various stages of mitosis.
Final answer b. Safranin
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