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Question

Which of the following step of translation does not consume a high energy phosphate bond?

A
Translocation
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B
Amino acid activation
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C
Peptidyl transferase reaction
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D
Aminoacyl tRNA binding to A site
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Solution

The correct option is B Peptidyl transferase reaction
A. Translocation is a kind of rearrangement in which a block of genes from one chromosome are transferred to other non homologous chromosome.
B. Amino acid activation is the addition of amino acid to tRNA and this reaction is catalyzed by aminoacyl tRNA synthase and ATP is used.
C. Peptidyl transferase reaction is catalyzed by the enzyme peptidyl transferase which forms a peptide bond between adjacent amino acids. This reaction doesn't consume a high energy phosphate bond, i.e. ATP or GTP.
D. Aminoacyl tRNA binds to A site of the larger ribosomal unit after activation of tRNA and in this reaction, elongation factor EF-G uses one GTP.
So, the correct answer is 'Peptidyl transferase reaction'.

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