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Question
Which of the following types of ELISA contain the following steps?
Antigen binding, Blocking, Primary antibody, Secondary antibody. Enzyme-linked antibody, Substrate, Colorimetric reading ( Represented in diagram).
Which of the following types of ELISA contain the following steps?
Antigen binding, Blocking, Primary antibody, Secondary antibody. Enzyme-linked antibody, Substrate, Colorimetric reading ( Represented in diagram).
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Solution
The correct option is D Sandwich ELISA
Sandwich ELISA is a less common variant of ELISA, but is highly
efficient in sample antigen detection. The sandwich ELISA quantify
antigens between two layers of antibodies. The antigen to be measured
must contain at least two epitopes capable of binding to two antibodies.
The basic steps involved are as follows:
(i) Plate is coated with capture antibody.
(ii) Sample is added, and any antigen present in the sample binds to capture antibody.
(iii) Detecting antibody is added, and binds to antigen.
(iv) Enzyme-linked secondary antibody is added that binds to detecting antibody.
(v) Substrate is added which gets converted by enzyme to detectable colour product that can be measured through colorimeter.
Sandwich ELISA is a less common variant of ELISA, but is highly efficient in sample antigen detection. The sandwich ELISA quantify antigens between two layers of antibodies. The antigen to be measured must contain at least two epitopes capable of binding to two antibodies. The basic steps involved are as follows:
(i) Plate is coated with capture antibody.
(ii) Sample is added, and any antigen present in the sample binds to capture antibody.
(iii) Detecting antibody is added, and binds to antigen.
(iv) Enzyme-linked secondary antibody is added that binds to detecting antibody.
(v) Substrate is added which gets converted by enzyme to detectable colour product that can be measured through colorimeter.
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