Question

Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?

• A. DNA can be seen in visible light
• B. DNA can be seen without staining in visible light
• C. Ethidium bromide stained DNA can be seen in visible light
• D. Ethidium bromide stained DNA can be seen under exposure to UV light

Answer 1

The correct option is D Ethidium bromide stained DNA can be seen under exposure to UV light

The separated DNA fragments (by the process of gel electrophoresis) are visible after staining the DNA with ethidium bromide followed by exposure to UV-radiation. These fragments are seen as orange coloured bands.