Question

Which one of the following statements is wrong with respect to separation of DNA fragments on gel electrophoresis?

• A. DNA fragments move towards anode under electric field through the matrix.
• B. The commonly used matrix is agarose gel.
• C. DNA fragments resolve according to their size.
• D. The smaller DNA fragments remain close to cathode.

Answer 1

The correct option is D The smaller DNA fragments remain close to cathode.

Solution : During the gel electrophoresis DNA fragments are separated on agarose gel. The electric field is applied and the DNA fragments as they are negatively charged moves towards the positive electrode or the anode. They are separated according to their sizes and the smaller ones are separated first and move towards anode.

So, the correct answer is option D.