Question

With regards to DNA extraction which of the following is/are correct?

• A. Only the purest DNA samples can be used in rDNA technology.
• B. DNA is present inside the nucleus of the cell which is embedded in the cytoplasm along with many organelles.
• C. DNA extraction increases the success of recombination.
• D. Since DNA extraction is easy, the step need not be avoided.

Answer 1

Answer: (A), (B)

DNA is present inside the nucleus of the cell which is embedded in the cytoplasm along with many organelles.

DNA is present in the nucleus of the cell along with several other organelles. It is also found to be closely associated with proteins especially histones and therefore they need to be removed for the recombinant DNA technology to be employed. For a successful recombinant DNA experiment, it is very important that the DNA sample is of utmost purity.