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Byju's Answer
Standard XII
Biology
Electroporation
Write any fou...
Question
Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombined technology experiments.
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Solution
Four ways to introduce the desired DNA segment into the bacterial host are:
Heat shock based transformation
Electroporation
Using disarmed bacteriophages as vectors (Transduction)
Micro-injection.
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Similar questions
Q.
Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombinant technology experiments.
Q.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombination DNA technology.
Q.
Given below are the steps of recombinant DNA technology.
(A) Introduction of desired gene into the host
(B) Identification of desired gene
(C) Transfer of desired gene to the progeny
(D) Isolation of desired gene
(E) Maintenance of introduced DNA into the host
Choose the correct sequence of events.
Q.
Recombinant DNA technology involves several steps in specific sequence. Find out the correct sequence.
a. Fragmentation of DNA
b. Culturing the host cells in a medium at large scale
c. Ligation of DNA fragment into a vector
d. Extraction of the desired product
e. Isolation of DNA
f. Isolation of desired DNA fragment
g. Transferring the recombinant DNA into the host
Q.
Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the break down of fungal cell, plant cell and bacterial cell respectively?
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