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Question

Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombinant technology experiments.

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Solution

The four ways used to introduce the desired DNA segment into a bacterial cell in recombinant technology experiments are
1.Microinjection
2.Heat shocking method
3.Gene-gun or biolistics method.
4.Using disarmed pathogen vectors.

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Q. Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombined technology experiments.

Q. Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the break down of fungal cell, plant cell and bacterial cell respectively?

Q. Given below are the steps of recombinant DNA technology.

(A) Introduction of desired gene into the host
(B) Identification of desired gene
(C) Transfer of desired gene to the progeny
(D) Isolation of desired gene
(E) Maintenance of introduced DNA into the host

Choose the correct sequence of events.

Q. Recombinant DNA technology involves several steps in specific sequence. Find out the correct sequence.

a. Fragmentation of DNA
b. Culturing the host cells in a medium at large scale
c. Ligation of DNA fragment into a vector
d. Extraction of the desired product
e. Isolation of DNA
f. Isolation of desired DNA fragment
g. Transferring the recombinant DNA into the host

Q. Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombination DNA technology.

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