Question

An analysis of chromosomal DNA using the southern hybridization technique does not use

• A. Autoradiography
• B. PCR
• C. Electrophoresis
• D. Blotting

Answer 1

The correct option is B

PCR

The correct option is B:

Explanation of the correct option:

1. Southern hybridization is a technique that requires transferring electrophoresis-separated DNA fragments to nitrocellulose carrier membranes and then using probe hybridization to detect the target DNA fragment.
2. The steps are as follows:
   1. DNA extraction and purification from living cells
   2. DNA Fragmentation Restriction or Digestion
   3. Electrophoresis for separation
   4. Denaturation
   5. Blotting
   6. Hybridization
   7. Autoradiograph
3. The southern hybridization approach does not require PCR to analyze chromosomal DNA.

Explanation of incorrect option:

Option A:

The southern hybridization approach does require Autoradiography to analyze chromosomal DNA.

Option C:

The southern hybridization approach does require Electrophoresis to analyze chromosomal DNA.

Option D:

The southern hybridization approach does require Blotting to analyze chromosomal DNA.

Final answer: The southern hybridization approach does not require PCR to analyze chromosomal DNA.