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Question

6CO2+12H2OLightC6H12O6+6H2O+6O2.
where C6H12O6 represents glucose. The O2 released is from water; this was proved using radio isotope techniques. Note that this is not a single reaction but description of a multistep process called photosynthesis. Can you explain why twelve molecules of water as substrate are used in the equation given?

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Solution

Products of light reaction required in dark reactions to form one molecule of glucose are 12 molecules of NADPH and 18 molecules of ATP.
For forming 2 molecules of NADPH from NADP+, 2 molecules of water are required so that demand of 4 electrons and protons can be satisfied.
Two molecules of water split to form 1 molecule of oxygen and the respective number of electrons and hydrogen ions are also formed.
So, for producing 12 molecules of NADPH, a total of 12 molecules of water are required.


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