Gene Silencing

Q. How is RNAi technology used to create pest resistant plants? [3]

Q. Silencing of a gene could be achieved through the use of:

1. Short interfering RNA only
2. Antisense RNA only
3. Both short interfering RNA and antisense RNA
4. None of these

Q.

What is RNA interference in biotechnology?

Q.

Is GFP a reporter gene?

Q. What are the advantages of transgenic crops?
1. Tolerant to abiotic stress
2. Nutritional enhancements
3. Increased pest management

1. only 1
2. only 2
3. 1, 2 and 3
4. only 2 and 3

Q.

Cry1 endotoxin obtained from Bacillus thuringiensis is effective against?

1. Nematodes

2. Flies

3. Mosquitoes

4. Boll worms

Q. Fruit softening is promoted by polygalacturonase which degrades

1. cellulose
2. protein
3. lipid
4. Pectin

Q.

What is RNA interference?

Q.

Enzyme polygalacturonase is responsible for

1. Vegetable softening

2. Fruit softening

3. Sugarcane rotting

4. Tomato hardening

Q.

E.coli is used in the production of

1. Rifampicin

2. LH

3. Interferon

4. Ecdysone

Q.

Enzyme polygalacturonase is responsible for

1. Vegetable softening

2. Fruit softening

3. Sugarcane rotting

4. Tomato hardening

Q. Double stranded RNA is cleaved by a nuclease called as Dicer and small fragments are generated that are known as _______.

1. short interspersed RNAs
2. long interspersed RNAs
3. small interfering RNAs
4. long interfering RNAs

Q.

What do prions look like?

Q. What is genetic RNA?

Q. ____________ of mRNA takes place during the process of RNAi.

1. Silencing
2. Transcription
3. Translation
4. Splicing

Q. RNA intereference is a mechanism for silencing gene expression at the

1. level of replication
2. level of transcription
3. post-transcriptional level
4. all the above

Q.

What is a natural process in which cells evolved to destroy RNA-based viruses?

Q. Identify the correct sequence of steps in RNA-interference (RNAi) process to develop nematode resistant plants.
i) Silencing of specific mRNA of the nematode
ii) Formation of sense and antisense RNA
iii) dsRNA formation
iv) Introducing nematode-specific genes into plant

1. i, ii, iv, iii
2. iii, ii, i, iv
3. iv, ii, iii, i
4. iv, i, ii, iii

Q. Statement I: Specific RNAi sequences are introduced into the cell which binds to and destroys its mRNA target.

Statement II: RNAi molecules can either increase or decrease a gene’s activity.

1. Only statement I is correct
2. Only statement II is correct
3. Both statements are correct
4. Both statements are incorrect

Q. What is euthenics ?

in both rna interference and antisense technology a nucleotide sequence binds to a m RNA .then what is the difference between thm

Q. What is the shape of T.M.V.? What is its genetic material?

Q. Which element is required for the synthesis of auxin?

1. Cu
2. Zn
3. Mo
4. Mn

Q. In RNAi, genes are silenced due to

1. ssDNA
2. dsDNA
3. dsRNA
4. ssRNA

Q. Which of the following correctly describes the function of RNase-H enzyme?

1. Causes RNA synthesis
2. Causes activation of hnRNA
3. Destruction of antisense nucleotide bounded mRNA
4. Causes reverse transcription of RNA

Q. Insect pest resistant, Bt-cotton plant was developed using

1. Somaclonal variation
2. Micropropagation
3. Somatic hybridization
4. Transgenic technology

Q. RNAi helps in producing pest resistant plants through the formation of

1. dsDNA
2. ssRNA
3. dsRNA
4. ssDNA

Q.

What is the other name given to RNA silencing?

Q. Very short answer type.
Name one ripening fruit, which produces ethylene gas.

Q. From which part of the cotton plant (Gossypium), maximum
cellulose fibre can be obtained

1. Stern surface
2. Leaf surface
3. Seed hair
4. Root hair

Q. The process of RNA interference has been used to make tobacco plant resistant to

1. Bacillus thuringiensis
2. Meloidogyne incognita
3. Files and mosquitoes
4. Both (a) and (b).